Seeing is Believing, and de novo protein structure determination will absolutely eliminate the guessing game. It is literally THE REASON why we look forward to coming to Icaria each day!
1. X-ray Crystallography
- Crystal screening and optimization (RockImager)
- Co-crystallization and ligand soaking
- X-ray data collection
- Structure determination (target or in complex)
- Structure interpretation
- Publication/deposit (SBIR, grant applications)
- Structure guided design (iterative operations)
- Fragment-based screening
- START packages of validated hits
- FBS Library access
- ~BioBlock
- ~Edelris
- ~Enamine
- ~Crystal First
- ~2Bind
2. Cryo-Electron Microscopy (Cryo-EM) Platform
- Negative Stain Screening (aggregation/homogeneity)
- Grid optimization and screening (Vitrobot)
- Low resolution data acquisition
- High resolution
- Data processing (particle picking, 2D classification, 3D classification, and 3D reconstruction)
3. Cryo-Electron Tomography (Cryo-ET) Platform
- Sample preparation by vitrification
- Localization by fluorescence
- Thinning by milling
- Electron cryo-tomography
- ~Visualization and structural analysis